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Identification of 3-indoleacetic acid in Pinus Sylvestris L. by gas chromatography - mass spectrometry and quantitative analysis by ion pair reversed phase LC with spectrofluorometric detection
Summary
Type:
HPLC
Application #:
106300
Catalog#:
720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection:
fluorescence, excitation 285nm, emission 360nm
Conditions:
eluent: 27.5% methanol in 0.01M phosphate buffer pH 6.5 containing 0.01M tetrabutylammonium hydrogen sulphate
flow rate 1.5ml/min
Authors:
Sandberg G
Source:
J Chromatogr 205 (1981) 125 - 137
Details
Legend: Fig. 3. Separation of indolecarboxylic acids by ion pair chromatography, 1. tryptophan, 2. 5-OH-IAA, 3. 3-indolelactic acid, 4. 3-indoleacrylic acid, 5. 3-indolepyruvic acid, 6. 3-indoleglyoxylic ac
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